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eGFP mRNA was diluted in 10 mM citrate buffer (pH 3) to obtain a mRNA: lipid weight ratio of 1:5

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Given the strong female predominance of AITD and the midlife rise in metabolic risk during the menopausal transition, targeted trials in perimenopausal and postmenopausal women are warranted, with attention to hormone status, body-composition change, cardiovascular risk, and dose adjustments of thyroid medications during weight loss [1,2,66] (Figure 2)