immobilized glutathione agarose An alternative easy method for antibody purification and analysis of protein–protein interaction using GST fusion proteins onto glutathione–agarose | Analytical and Bioanalytical Chemistry columns and binding Glutathione Agarose | MedChemExpress

doi: 10.1016/j.nut.2012.01.005

The samples were then placed in a VACUETTE tube and stored at a temperature of 4 C for a duration of 30 min

System X c System X c , a crucial negative regulator of ferroptosis, structurally consists of the twelve-pass transmembrane transport protein cystine/glutamate transporter (SLC7A11) and the single-pass transmembrane regulator protein 4F2 cell-surface antigen heavy chain (SLC3A2), and is responsible for maintaining redox homeostasis by importing cystine to synthesize GSH [128, 129]

Soejima, H

doi:10.1001/jamaoto.2019.4312 Jung SY, Jung J, Byun JY, Park MS, Kim SH, Yeo SG

SLC7A11 deficiency accelerates the progression of high-fat diet-induced MASLD through ferroptosis induced by classical cystine/cysteine deficiency, while serine deficiency and consequent obstruction of de novo synthesis of cysteine are responsible for the progression of ferroptosis induced MAFLD in hepatic SLC7A11 overexpressing mice