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Proteomics 8, 301315

Where they exist, sample sizes tend to be small, follow-up periods are short, and dosing protocols vary

HEK293T cells were transfected with 2 g pX330-SHARP 3gRNA and 4 g plasmid repair template comprising EGFP-P2A-puromycin and flanking 1 kb homology arms for SHARP-GFP or 2 g pX330-SHARP 3gRNA + 2 g pX330-SHARP SPOC gRNA to excise the region encoding SPOC and 4 g plasmid repair template for SHARP SPOC-GFP

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The maximum absorbance max was obtained at 290 nm, and then the calibration curve was generated to examine further characterizations

doi: 10.3390/biomedicines10092087