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These cultures were incubated at 37C with agitation until reaching an OD 600 of 0.8-1.0 for the induction of protein expression with 0.2 mM IPTG at 16C for 16 hours
[Google Scholar] 128.Sharma M., Khan S., Rahman S., Singh L.R

Furthermore, steady cavitation and acoustic streaming facilitate passive diffusion and augment local fluid transfer [235]

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