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Briefly, after enrichment, FoB were washed and resuspended in mannitol and sucrose-BSA (MAS-B) buffer pH 7.2 (70 mM sucrose, 220 mM Mannitol, 10 mM KH 2 PO 4 , 5 mM MgCl 2 , 2 mM Hepes, 1 mM EGTA, and 0.4% fatty acid-free BSA), and flux measurements were started

This method is suitable for hydrophilic drugs but often yields low encapsulation efficiency due to the large volume of external aqueous phase

Early intervention with B12 injections helps restore balance and prevents lasting health consequences, making proactive care essential

Minor particles: pass the solution through a sterile 0.2 micron laboratory filter

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