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Given the established role of the BAX 1 region in BAX activation 28,42,43 and the evidence of B-017 binding in the vicinity of helix 1, as revealed by cross-linking analysis, we conducted an in vitro study to investigate the impact of B-017 on BAX activation

Loss of GPX4 activity or depletion of GSH and inhibition of cysteine uptake leads to increased lipid peroxidation and ferroptosis [33]

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The dialysis bag filled with nanoparticles was placed in a PBS solution (pH7.4) with or without GSH (5 mM or 10 mM)

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These results provide the first in vivo evidence that intranasal NAD administration may be a novel strategy for decreasing brain damage in cerebral ischemia