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Dihydrofolate reductase (DHFR) which catalyzes the conversion of dihydrofolate into tetrahydrofolate, the active form of folic acid, is competitively inhibited by MTX and MTX-PGs, which depletes vital tetrahydrofolate (THF) needed for cellular functions (Xu et al

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Similarly, freezing reconstituted peptide destroys its structure

Longer continuous use (months) departs from the cycling framework and has no specific evidence base

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Ghanizadeh A, Mohammadi MR, Bahraini S et al (2017) Efficacy of N-acetylcysteine augmentation on obsessive compulsive disorder: a multicenter randomized double blind placebo controlled clinical trial