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Collagen synthesis requires amino acids, particularly glycine, proline, and hydroxyproline

You can read more in our guide to bacteriostatic water for peptides

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Skin improvements persist for weeks to months after the protocol ends, reflecting the time required for collagen turnover to return to baseline rates

This means dividing the full reconstituted volume into several smaller, single-dose amounts in separate sterile vials or syringes and then freezing them