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For SuperScript IV (SSIV), the 20 L reaction contained 4 L 5 SSIV buffer (Thermo Fisher Scientific), 4 L 100 mM DTT, 1 L RNase OUT inhibitor (Thermo Fisher Scientific), 1 L SSIV reverse transcriptase, and 10 L annealed RNA

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Several factors that promote BBB access have been suggested to participate in the formation of such antibodies

Guided by these results, we tested the outgrowth of cholangiocyte organoids in collagen I hydrogels, directly from primary liver biopsies

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