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Consequently, in prokaryotic Atm1 proteins, a more electroneutral surrounding is present, including two methionines at the inner gate, NaAtm1 M317 and M320 of TM6, that directly links to the cargo, and even to the mercury atom of GS-Hg, in agreement with HSAB Pearson theory 37 , postulating that many heavy metals rely on soft ligand coordination by methionines and cysteines (Supplementary Fig