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Enzyme assays Preparation of enzyme extract After grounding the basil leaves (0.3 g) and suspending them in 3 mL of 25 mM ice-cold Hepes buffer with pH 7.8, containing EDTA (0.2 mM), reduced ascorbate (2 mM), and 2% polyvinylpyrrolidone, the mixture was centrifuged (12,000 rpm-20 min-4 C) and the supernatant was used for determining the enzyme activity 15
