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10.1093/oxfordjournals.humrep.a136893 23

Mouse Tumor Cell Isolation, Fluorescence-Activated Cell Sorting and RNA Sequencing p48-Cre/p53F/+KrasL/+/ROSA26-LSL-YFP ( Sirt6 cKO), as well as Sirt6 WT p48-Cre/p53F/+KrasL/+/ROSA26-LSL-YFP as well as 4T1-eGFP tumor bearing mice were euthanized and entire tissues (primary tumors, livers and lungs) were minced and incubated with 1.3mg/ml collagenase (Sigma-Aldrich, C6079) on a horizontal shaker at 37 C for 30 min, vortexing every 5 min

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Orlandi A, Bennett M