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Subsequently, a 2% (v/v) inoculum (2 mL) was used to inoculate a fresh 100 mL TSB broth, which was then incubated for an additional 5 h at 37 C to reach the logarithmic phase

Diagnostic methods Glutathione and sulphydryl compunds in erythrocytes can be measured using 5,5'-dithiobis (2-nitrobenzoic acid), while glutathione in fibroblasts can be measured with the 5,5'-dithiobis (2-nitrobenzoic acid) glutathione recycling assay [16], or using high performance liquid chromatography (HPLC) [17]

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In this way, GSH performs in both metal homeostasis and the antioxidative defense, which influence the levels of free reduced GSH and its cellular redox state [ i.e

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