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In this scenario, you would expect all of your protein to be in the elution, and none of it to be in the flow-through

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Pantothenic acid Pantothenic acid (vitamin B 5 ) is required as a component of coenzyme A (CoA), a coenzyme needed for the oxidative metabolism of glucose and fatty acids and for the biosynthesis of fatty acids, cholesterol, steroid hormones, the hormone melatonin, and the neurotransmitter acetylcholine